Moligo knowledge
Five questions that separate suppliers.
Ask how the product is made, what is measured and which limits apply to your construct.
Make the specification reviewable.
| Ask | Why it matters | What an incomplete answer looks like | Moligo’s answer |
|---|---|---|---|
| Which polymerase and how many effective doublings? | Process history affects variant burden. Routine size purification does not certify sequence identity. | An enzyme fidelity figure offered as a finished-product variant rate. | Isothermal rolling-circle amplification from a sequence-verified template. Product-level variant assessment and process details: agreed per project. |
| Was ssDNA produced directly or recovered from duplex material? | The route determines which intermediates and carryover need measuring. | “Not detectable” without an assay or detection limit. | Strand-production and recovery details: agreed per project. |
| How much residual dsDNA, of what size, measured how? | Duplex content and size can affect biological interpretation. Assay sensitivity must be stated. | A gel image without a validated limit of detection. | Published service QC includes gel and nuclease testing. Numerical residual dsDNA limits and size-distribution methods: agreed per project. |
| Where does the chemistry sit? | Two 25-base adaptors span 1% of a 5,000-base donor by length arithmetic. | “Modified DNA” without location, chemistry or fraction. | Global substitution with per-base-channel dose control. Incorporation is tunable. Product composition and feasibility: agreed per project. |
| Is circular ssDNA available, and how is format purity measured? | Topology is a separate design variable from chemical composition. | A purity percentage without an assay. | Linear and circular ssDNA services. Published circular-format checks include S1 and exonuclease challenges. Quantitative format-purity specification: agreed per project. |
Adaptor arithmetic assumes two 25-base adaptors on a 5,000-base donor. Analytical requirements vary by construct and use. See Moligo’s published service QC criteria.