Circular ssDNA in non-viral gene insertion.
Moligo and Cellectis co-authored research in Nature Communications on circular ssDNA donors in HSPCs. Circular ssDNA was three to five times more efficient than linear for knock-in, worked at multiple loci and in primary T cells, and cells edited with circular ssDNA showed greater engraftment capacity than cells edited using AAV6 in mouse models.
Moligo co-authors disclose employment and equity holdings.
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“This study provides clear evidence that Moligo’s enzymatically produced long ssDNA vectors can serve as a robust, scalable alternative to viral delivery.”Cosimo Ducani, CEO, Moligo Technologies
“These results establish the CssDNA and TALEN® editing process as an efficient non-viral gene insertion strategy.”Julien Valton, VP, Cellectis